[Histonet] Frozen brains on cryostat

[Emily Sours]

If they haven't been fixed, good luck! I've never been able to section
unfixed tissue.
Then again, I work with embryonic tissue only.
Have you tried sectioning at a smaller section size?

Emily



The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson




On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) <
aferullo <@t> celgene.com> wrote:

> Hello everyone,
>
> I recently received rat brain samples that were frozen in liquid nitrogen
> and embedded in OCT.  I sectioned them on the cryostat and they are coming
> out very wrinkled, no matter what technique I use to pick them up.  I would
> appreciate any tips/tricks  that anyone has to offer.  Forebrain sections
> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time.
>  Thanks and I look forward to your advice.
>
> Andrea
>
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