[Histonet] RE: Gram Stain

[Tony Henwood (SCHN)]

Stacy,

There are several Gram stain variants that do not use Picric acid. 

Tworts variant uses fast green and neutral red  after the crystal violet-Iodine steps.

Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5% Tartrazine after the crystal-violet steps, to stain the gram negative bugs.

A simple red counterstain is to stain sections with 1% aqueous neutral red for 3 min after the crystal violet-Iodine steps

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Giroux, Stacy
Sent: Tuesday, 13 March 2012 4:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gram Stain

Hi,

Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good?

Thank you for your help,
Stacy




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