[Histonet] Gram Stain

[gayle callis]

Stacy, 

You can buy a complete Brown and Brenn staining kit from Newcomer Supply
which is very good, and not change your method.   The picric acid/acetone
mixture is included and you wouldn't have to store stock picric acid nor
ether in the lab but just this small amount of Picric acid/acetone that
comes in the kit.  Disposal should not be that much of a problem.   They
also have a Hucker Twort Gram stain that uses acetone for destaining and you
supply the acetone.  Poly Scientific has Gram Stain kit (Hucker
modification) which uses Grams decolorizer, a mixture of acetone and
alcohol.  They have excellent staining kits too.       

Gayle Callis
HTL/HT/MT(ASCP)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Giroux,
Stacy
Sent: Monday, March 12, 2012 11:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gram Stain

Hi,

Our lab is currently transitioning from the Brown & Brenn gram stain due to
no longer wanting to store picric acid due to its potential hazards. Our
pathologists have requested a gram stain for paraffin embedded tissue that
looks similar but does not use picric acid or ether. Does anyone have any
suggestions on stains that could be used or gram stain kits that are
available for purchase that are good?

Thank you for your help,
Stacy




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