[Histonet] Goldner's Trichrome for Osteoid

[Grantham, Andrea L - (algranth)]

Hopefully a hard tissue guru is out there and can help with this.

A PI here is asking about doing a Goldner's stain on mouse femur. I'm not set up to cut sections of undecalcified bone so my questions are:
1. can this stain be done successfully on decalcified bone?
2. if so, what type of decal should be used - EDTA?
3. I have googled the stain but didn't see what the preferred thickness was for this stain.

Need this info fairly soon! The bones are on the way.

Thanks!!! 

Andi G.