[Histonet] Goldner's Trichrome for Osteoid

[Louise Renton]

As far as i know, osteoid will not show up on  a decalcified section.

On Thu, Mar 8, 2012 at 6:00 PM, Grantham, Andrea L - (algranth) <
algranth <@t> email.arizona.edu> wrote:

> Hopefully a hard tissue guru is out there and can help with this.
>
> A PI here is asking about doing a Goldner's stain on mouse femur. I'm not
> set up to cut sections of undecalcified bone so my questions are:
> 1. can this stain be done successfully on decalcified bone?
> 2. if so, what type of decal should be used - EDTA?
> 3. I have googled the stain but didn't see what the preferred thickness
> was for this stain.
>
> Need this info fairly soon! The bones are on the way.
>
> Thanks!!!
>
> Andi G.
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
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