[Histonet] Smad4 (B-8) / DPC4

[Angela Bitting]

I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know.
 
Angie
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 


>>> "Giroux, Stacy" <Stacy.Giroux <@t> stjohn.org> 3/6/2012 7:35 AM >>>
Hi,

I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated.

Thanks,
Stacy


Stacy Giroux, HTL(ASCP)
Operations Coordinator, Histology
St. John Hospital & Medical Center
Phone: 313-343-3130
Fax: 313-343-4965

Histotechnology Professionals Day - March 10, 2012


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