[Histonet] Smad4 (B-8) / DPC4

Patrick Laurie foreightl <@t> gmail.com
Tue Mar 6 12:43:04 CST 2012


Our SMAD-4 is run on the Biocare Intellipath using the Santa Cruz B-8
clone.  We do Diva retrieval, 1:200 dilution for 30 minutes followed
by PeroxAbolish to eliminate non-specific background staining.  Our
pathology group has been happy with this.

On Tue, Mar 6, 2012 at 5:34 AM, Angela Bitting <akbitting <@t> geisinger.edu>wrote:

> I've tried to work it up on the XT also with inconsistent, weak results.
> If you get a reply on this one, let me know.
>
> Angie
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
>
>
>
> >>> "Giroux, Stacy" <Stacy.Giroux <@t> stjohn.org> 3/6/2012 7:35 AM >>>
>  Hi,
>
> I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are
> currently trying to work up this antibody for validation; however, we are
> having significant problems getting it to work consistently. We are using
> the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If
> anyone has protocol information to share regardless of platform used it
> would be greatly appreciated.
>
> Thanks,
> Stacy
>
>
> Stacy Giroux, HTL(ASCP)
> Operations Coordinator, Histology
> St. John Hospital & Medical Center
> Phone: 313-343-3130
> Fax: 313-343-4965
>
> Histotechnology Professionals Day - March 10, 2012
>
>
> CONFIDENTIALITY NOTICE:
> This email message and any accompanying data or files is confidential and
> may contain privileged information intended only for the named
> recipient(s). If you are not the intended recipient(s), you are hereby
> notified that the dissemination, distribution, and or copying of this
> message is strictly prohibited. If you receive this message in error, or
> are not the named recipient(s), please notify the sender at the email
> address above, delete this email from your computer, and destroy any copies
> in any form immediately. Receipt by anyone other than the named
> recipient(s) is not a waiver of any attorney-client, work product, or other
> applicable privilege._______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> IMPORTANT WARNING: The information in this message (and the documents
> attached to it, if any) is confidential and may be legally privileged. It
> is intended solely for the addressee. Access to this message by anyone else
> is unauthorized. If you are not the intended recipient, any disclosure,
> copying, distribution or any action taken, or omitted to be taken, in
> reliance on it is prohibited and may be unlawful. If you have received this
> message in error, please delete all electronic copies of this message (and
> the documents attached to it, if any), destroy any hard copies you may have
> created and notify me immediately by replying to this email. Thank you.
>
> Geisinger Health System utilizes an encryption process to safeguard
> Protected Health Information and other confidential data contained in
> external e-mail messages. If email is encrypted, the recipient will receive
> an e-mail instructing them to sign on to the Geisinger Health System Secure
> E-mail Message Center to retrieve the encrypted e-mail.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com


More information about the Histonet mailing list