[Histonet] Smad4 (B-8) / DPC4

[Patrick Laurie]

Our SMAD-4 is run on the Biocare Intellipath using the Santa Cruz B-8
clone.  We do Diva retrieval, 1:200 dilution for 30 minutes followed
by PeroxAbolish to eliminate non-specific background staining.  Our
pathology group has been happy with this.

On Tue, Mar 6, 2012 at 5:34 AM, Angela Bitting <akbitting <@t> geisinger.edu>wrote:

> I've tried to work it up on the XT also with inconsistent, weak results.
> If you get a reply on this one, let me know.
>
> Angie
>
> Angela Bitting, HT(ASCP), QIHC
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
>
>
>
> >>> "Giroux, Stacy" <Stacy.Giroux <@t> stjohn.org> 3/6/2012 7:35 AM >>>
>  Hi,
>
> I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are
> currently trying to work up this antibody for validation; however, we are
> having significant problems getting it to work consistently. We are using
> the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If
> anyone has protocol information to share regardless of platform used it
> would be greatly appreciated.
>
> Thanks,
> Stacy
>
>
> Stacy Giroux, HTL(ASCP)
> Operations Coordinator, Histology
> St. John Hospital & Medical Center
> Phone: 313-343-3130
> Fax: 313-343-4965
>
> Histotechnology Professionals Day - March 10, 2012
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com