[Histonet] Re: Tolivia, Navarro and Tolivia

Shanon Pink shanonpink <@t> gmail.com
Mon Jun 25 08:55:13 CDT 2012

Hi Again Gayle,

I'm sorry I was not very clear; I have already downloaded and read the
publication, and I am trying the procedure out today. I was wondering
about using a couple of substitutions and thought I might ask if
anyone has tried this procedure before.

I am on a four-week research project trying to find a nice
polychromatic stain to use for epon-embedded thick sections for
researchers here at the EM lab at St. Jude Children's Research
Hospital in Memphis. Normally they just use Toluidine Blue, but in
some cases researchers would like to have better differentiation on
their thick sections. Initially we were trying to find a stain that
approximates H&E since that's a familiar stain, but I've only had
mediocre results with the following stains: (1) Toluidine Blue/Sodium
Borate, followed with Basic Fuchsin, and (2) a homemade Paragon stain.
The staining procedure described in the aforementioned publication is
performed at  room temperature and is relatively fast. But as you
point out, it involves phenol which is not fun to work with.

Thanks to you and anyone else who has any thoughts!

--Shanon Pink

On Mon, Jun 25, 2012 at 8:42 AM, gayle callis <gayle.callis <@t> bresnan.net> wrote:
> Shanon,
> Thank you.    If you are at a university, you should be able to get this
> publication on line if their library can do this for you.   I am not sure I
> can access it via my connections but will try later.   There are other
> methylene blue/basic fuchsin protocols that work very well on epoxy
> sections.  Keep in mind that the carbol component of this stain is just
> another name for phenol, very toxic and often a controlled substance in
> laboratories (can't be passed around!).
> If you want I will go into my EM file for the other MB/BF methods, they are
> simple to use and make up.   Let me know if you would like the publication.
> Gayle Callis
> -----Original Message-----
> From: Shanon Pink [mailto:shanonpink <@t> gmail.com]
> Sent: Monday, June 25, 2012 6:40 AM
> To: gayle callis; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: Tolivia, Navarro and Tolivia
> My apologies Gayle, I am new at this...
> >From Histochemistry (1994) 101:51-55, "Polychromatic staining of epoxy
> semithin sections: a new and simple method," by Tolivia, Navarro and
> Tolivia. I am wondering if anyone out there has tried this technique.
> Thank you for your time in responding!
> --Shanon
> On Fri, Jun 22, 2012 at 3:47 PM, gayle callis <gayle.callis <@t> bresnan.net>
> wrote:
>> What  journal, year and issue for this publication?
>> ********************
>> You wrote:
>> Has anyone out there tried the staining procedure described in the
>> paper "Polychromatic staining of epoxy semithin sections: a new and
>> simple method," from 1994, by Tolivia, Navarro, and Tolivia? I would
>> like to try it out on some thick sections embedded in Epon, but I
>> can't find any carbol methylene blue and wondered about possible
> substitutions.
>> Gayle Callis

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