[Histonet] Frozen Sections Falling Off Slides

[Kim Donadio]

Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC. 

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On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzrosari <@t> yahoo.com> wrote:

> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland...
> I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised  please.Maybe youre using the same tissue tek cryo 3...
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