[Histonet] Fluorescence-filters

Anatoli Gleiberman AGleiberman <@t> cbiolabs.com
Tue Jun 5 11:39:42 CDT 2012

Filters are usually very stable. What is burning out are fiber optic cables that are present in some illumination systems such as X-Cite in Zeiss microscopes. These optical fiber cables should be replaced after 2000 hours.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, June 05, 2012 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fluorescence-filters


Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year.


What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. 


Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.


Hoping for responses

Gudrun Lang

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