[Histonet] Fluorescence-filters -2

[Gudrun Lang]

I forgot: the filters are now 10 years old.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Dienstag, 05. Juni 2012 16:38
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Fluorescence-filters

Hi!

Filters for fluorescencemicroscopy tend to "burn out" after a certain
duration of usage. What duration?

We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
are about 150 per year.

 

What do you think? Is it time to change them. 

I have often bad feedback about weak signals, and I would not be surprised
if the microscope is the culprit and not our protocol. 

 

Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but
tumourcells mixed within collagenfibers. 

- and unfortunately unexperienced doctors on reading of this special probe.

 

Hoping for responses

Gudrun Lang

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