[Histonet] Sections washing off slides

[Mehlika Faire]

Have you tried baking or placing your slide on a slide warmer for a few hrs or overnight prior to staining? That seems to help make the sections stick.I would do this step after transferring the section to a charged slide.

-Mehlika 

> From: kstoll <@t> mcw.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 27 Jul 2012 19:47:40 +0000
> Subject: [Histonet] Sections washing off slides
> 
> I have some old H&E slides that I would like to try IHC staining on.  I am having trouble keeping the tissue on the slide.
> I put a slide in xylene to remove the cover slip and eventually the section floated off the slide when I tried to rehydrate.
> The second time I tried Mount-Quick from Newcomer Supply and was able to put the section on a charged slide.  The section stayed on the slide but was loose after hydration.   It was just hanging on after retrieval and did not stain well.
> I have a limited number of slides.  If anyone has experience in this area and knows the best way to keep tissue on a slide I would really appreciate your advice.  Thanks in advance.
> 
> Kathryn Stoll, HT(ASCP)
> Department of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
> 
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