[Histonet] counter stain for PAS for fungus
Jennifer MacDonald
jmacdonald <@t> mtsac.edu
Mon Jul 23 15:08:11 CDT 2012
Add some acetic acid to the fast green.
On Jul 23, 2012, at 9:11 AM, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
> Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus. The fast green stain is simply not staining anymore no matter how long I leave it on the specimen. Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows:
> Periodic Acid 7 min
> quick rinse
> Schiff solution 20min
> wash for 10min
> Fast green stain 1 min
> Any help is appreciated.
> Thanks in advance,
> Michele Carr
> Medical Laboratory Services
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