[Histonet] counter stain for PAS for fungus

[Rena Fail]

I have used a working solution light green Sf yellowish C,I. # 42095 and
the only time I had problems was when someone left out the glacial acetic
acid in the stock,

Stock is light green SF yellowish 0.2 gm,distilled water 100.0 ml, 0.2 ml
glacial acetic acid.
Working  is 10.0 ml of stock, 50.0 ml  distilled water

Rena Fail

On Mon, Jul 23, 2012 at 12:11 PM, Michele Carr <michelecarr10 <@t> yahoo.com>wrote:

> Hi everyone I am having a problem with the fast green counterstain for the
> PAS for fungus.  The fast green stain is simply not staining anymore no
> matter how long I leave it on the specimen.  Do you have any suggestions on
> how to correct this or perhaps recommendations for a different
> counterstain. Our protocol is as follows:
> Periodic Acid 7 min
> quick rinse
> Schiff solution 20min
> wash for 10min
> Fast green stain 1 min
>  Any help is appreciated.
> Thanks in advance,
> Michele Carr
> Medical Laboratory Services
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