[Histonet] counter stain for PAS for fungus

[Rene J Buesa]

Prepare a fresh solution
René J.


________________________________
From: Michele Carr <michelecarr10 <@t> yahoo.com>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, July 23, 2012 12:11 PM
Subject: [Histonet] counter stain for PAS for fungus

Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus.  The fast green stain is simply not staining anymore no matter how long I leave it on the specimen.  Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows:
Periodic Acid 7 min
quick rinse 
Schiff solution 20min
wash for 10min
Fast green stain 1 min
 Any help is appreciated.
Thanks in advance,
Michele Carr 
Medical Laboratory Services
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