[Histonet] How to make HP control tissue?

[Mark Tarango]

Hi Willem,

H. pylori needs the low pH of the stomach to survive and grow.  It won't
naturally be in lung tissue.  When looking for HP, it's found on the mucosa
and gastric pits of the stomach.  Since you're looking for it in a specific
place, I think putting it in lung is a bad idea.

Your best bet for control tissue is a positive gastrectomy specimen.  You
could chop that up into a thousand pieces and never run out.  We've had
several cases like this.

Send me your address and a FedEx account number and I'll see about send you
a block or two.

Mark

On Wednesday, July 4, 2012, Hoekert, W.E.J. wrote:

> Hi Histonetters,
>
> Does anybody has experience in making your own HP control tissue? I am
> tired of using positive biopsies of patients since they are always almost
> finished.  I have heard of a procedure on making HP controls but I am not
> exactly sure how it is done.
>
> I have tried the following: I went to the microbiology department and
> asked for some freshly grown HP bacteria. We scraped them of the
> petridishes and put them in formalin. I had them fixed for 24 hours and
> then I injected them into some already fixed lung tissue and processed it
> as normal. But if I do a HP stain on that tissue, I don't see any bacteria.
> Probably they rinse off during the process.
>
> Can anyone tell me how to do it?
>
> Thanks in advance,
>
> Willem Hoekert
> OLVG
> The Netherlands
>
>
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