[Histonet] Frozens and antigen retrieval

[Kim Donadio]

You should not have to use retreival. Retrieval either by heat/solution or enzyme digestion is usually needed when the antigen/antibody site has been blocked/masked by the fixation process.
 
Kim D 


________________________________
From: Daniela Bodemer <daniela.bodemer <@t> mcri.edu.au>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, July 4, 2012 7:02 AM
Subject: [Histonet] Frozens and antigen retrieval

Hi all,

A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no?

The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. 

Hit me with your opinions on this :-)

Thanks in advance,

Daniela

Sent from my iPad

______________________________________________________________________
This email has been scanned by the Symantec Email Security.cloud service.
For more information please visit http://www.symanteccloud.com
______________________________________________________________________

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet