[Histonet] How to make HP control tissue?

[William]

It may be hard to find someone to help you in the States. Doing that is against regulations. We must use human tissue with a natural infection I we are going to be using it as a control for human medical testing.  That being said, you are right, your hp is washing out of the tissue.

 My suggestion is to not fix the hp culture, inject it fresh into unfixed tissue. Preferable fresh autopsy stomach or intestine. Allow it to culture (and attach) for about an hour or 2 and then fix. Be careful too long out of fixative and autolysis will occur.  I hope that works.

I have no experience with lung. It should work, but I do not know if the environment would cause the bacteria to attach and grow. 

Will

Sent from my iPhone

On Jul 4, 2012, at 11:09 AM, "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl> wrote:

> Hi Histonetters,
> 
> Does anybody has experience in making your own HP control tissue? I am tired of using positive biopsies of patients since they are always almost finished.  I have heard of a procedure on making HP controls but I am not exactly sure how it is done.
> 
> I have tried the following: I went to the microbiology department and asked for some freshly grown HP bacteria. We scraped them of the petridishes and put them in formalin. I had them fixed for 24 hours and then I injected them into some already fixed lung tissue and processed it as normal. But if I do a HP stain on that tissue, I don't see any bacteria. Probably they rinse off during the process. 
> 
> Can anyone tell me how to do it? 
> 
> Thanks in advance,
> 
> Willem Hoekert
> OLVG 
> The Netherlands
> 
> 
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