[Histonet] How to make HP control tissue?

[Hoekert, W.E.J.]

Hi Histonetters,
 
Does anybody has experience in making your own HP control tissue? I am tired of using positive biopsies of patients since they are always almost finished.  I have heard of a procedure on making HP controls but I am not exactly sure how it is done.
 
I have tried the following: I went to the microbiology department and asked for some freshly grown HP bacteria. We scraped them of the petridishes and put them in formalin. I had them fixed for 24 hours and then I injected them into some already fixed lung tissue and processed it as normal. But if I do a HP stain on that tissue, I don't see any bacteria. Probably they rinse off during the process. 
 
Can anyone tell me how to do it? 
 
Thanks in advance,
 
Willem Hoekert
OLVG 
The Netherlands
 

Disclaimer: 

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.