[Histonet] Factor XIIIa background staining problems

[Leslie Apa]

Hi Jason,

I have been working with Janna on this issue and just got her email that The problem persists even after my suggestions. I can assure you the expiry is fine, many time the antibodies are still stable and usable even after the expiry date.  Have you tried a variety of controls from different blocks? What is the lot number of this antibody? I will look further into this and see what else I can suggest. I may want to send you a couple stained and unstained slides to see if you can get staining on our tissue.  We recommend a 1:250 dilution but that being said if you are still experiencing the background you have the flexibility of increasing the dilution to see if that helps. I will get back to you as soon as I can, but will need the lot number to check the device history.

Thanks you,

Leslie Apa
Technical Consultant
916-746-8900 office
605-580-5333 mobile
Sent from my iPhone

On Jan 31, 2012, at 12:33 PM, "Jason McGough" <jmcgough <@t> clinlab.com> wrote:

> We have been experiencing Factor XIIIa background staining problems and
> cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa
> clone EP3372 at a dilution of 1:250. We have tried many different protocols
> (i.e. enzyme pretreatment, high and low pH pretreatment, different
> incubation times, etc.)and nothing seems to fix this background staining.
> Our vendor told us that the dilution is good but we are questioning the
> actual dilution or the shelf life of the antibody. The antibody is a long
> ways from reaching the expiration date but we are still questioning it's
> stability. Has anybody else experienced this or know a solution?
> 
> Thanks,
> 
> Jason McGough HT(ASCP)
> Account Representative - Anatomic Pathology
> Clinical Laboratory of the Black Hills
> 2805 5th Street Suite 210
> Rapid City, SD 57701
> 605-343-2267 Ext 127
> 605-718-3779 (Fax)
> jmcgough <@t> clinlab.com
> 
> 
> 
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