[Histonet] Factor XIIIa background staining problems

[Elizabeth Chlipala]

I would look at the antibody dilution and redo your titer study, your primary antibody is probably too concentrated.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jason McGough
Sent: Tuesday, January 31, 2012 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Janna Hope
Subject: [Histonet] Factor XIIIa background staining problems

We have been experiencing Factor XIIIa background staining problems and
cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa
clone EP3372 at a dilution of 1:250. We have tried many different protocols
(i.e. enzyme pretreatment, high and low pH pretreatment, different
incubation times, etc.)and nothing seems to fix this background staining.
Our vendor told us that the dilution is good but we are questioning the
actual dilution or the shelf life of the antibody. The antibody is a long
ways from reaching the expiration date but we are still questioning it's
stability. Has anybody else experienced this or know a solution?

Thanks,

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com



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