[Histonet] clostridium and bacterial spores in tissue sections

[Louise Renton]

I used to flame tissue sections regularly when doing a ZN for TB...a wisp
of cotton wool held in forceps, dipped in alcohol and lit with  a match. A
few quick passes under the slide and bob's yr uncle. worked fine, until the
day came that I ignited some acetone fumes in the sink and fireballed my
eyebrows off!

Gentle heating in a incubator also works

On Sat, Jan 14, 2012 at 8:34 PM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> When I was in pre-medical (59 years ago, imagine!) we used the same stain
> you are referring to that involved Ziehl-Nielsen and light green as counter
> stain with heat, so much heat, that a flame has to be applied to dry the
> smears.
> In Peter Gray's book there are about 40 methods described,none for tissues.
> I am not sure a tissue section will survive, but, why not, just try it,
> and let us all know.
> René J.
>
> --- On Sat, 1/14/12, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:
>
>
> From: Elizabeth Chlipala <liz <@t> premierlab.com>
> Subject: [Histonet] clostridium and bacterial spores in tissue sections
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Date: Saturday, January 14, 2012, 12:58 PM
>
>
> Hello everyone
>
> I need a bit of help.  I have to stain for clostridium and bacterial
> spores in tissue sections.  I have come across a few references to staining
> these on smears but not on tissue sections.  I did find one reference that
> used Ziehl-Neelsen to stain terminal spores from clostridium tetani.  Does
> anyone out there know if the stain they use in microbiology for smears will
> work on tissue sections?  Any advice would be appreciated.
>
> Thanks in advance
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308-1592
> (303) 682-3949 office
> (303) 682-9060 fax
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> www.premierlab.com<http://www.premierlab.com>
>
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
Question: Are rhinos  overweight unicorns?