[Histonet] clostridium and bacterial spores in tissue sections

Richard Cartun Rcartun <@t> harthosp.org
Mon Jan 16 08:30:09 CST 2012

Hi Liz:

I have used IHC to identify Clostridia on formalin-fixed tissue.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> Elizabeth Chlipala <liz <@t> premierlab.com> 1/14/2012 12:58 PM >>>
Hello everyone

I need a bit of help.  I have to stain for clostridium and bacterial spores in tissue sections.  I have come across a few references to staining these on smears but not on tissue sections.  I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani.  Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections?  Any advice would be appreciated.

Thanks in advance


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
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(303) 881-0763 cell

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