[Histonet] clostridium and bacterial spores in tissue sections

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Jan 14 12:34:59 CST 2012

When I was in pre-medical (59 years ago, imagine!) we used the same stain you are referring to that involved Ziehl-Nielsen and light green as counter stain with heat, so much heat, that a flame has to be applied to dry the smears.
In Peter Gray's book there are about 40 methods described,none for tissues.
I am not sure a tissue section will survive, but, why not, just try it, and let us all know.
René J.

--- On Sat, 1/14/12, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:

From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] clostridium and bacterial spores in tissue sections
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, January 14, 2012, 12:58 PM

Hello everyone

I need a bit of help.  I have to stain for clostridium and bacterial spores in tissue sections.  I have come across a few references to staining these on smears but not on tissue sections.  I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani.  Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections?  Any advice would be appreciated.

Thanks in advance


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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