[Histonet] difficult cross section to cut

[Rittman, Barry R]

Louise
A product that used to work well for me in the middle ages was called "Mollifex" came from BDH.
Barry

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton <@t> gmail.com]
Sent: Wednesday, January 11, 2012 1:37 AM
To: Histonet
Subject: Re: [Histonet] difficult cross section to cut

Hi Peggy
1. I have found that to cut  bone the block has to be REALLY cold. Face
block with increased knife angle as suggested by Rene, and then place in
freezer overnight - then try sectioning
2. Try surface decalcification of your block overnight in whichever soln.
you normally use.
3. Section at 4-6 microns
4. You do not say, but high profile disposables work better with bone than
low profile
5, failing this, you could try dewaxing and re decalcifying for a few more
days

good luck



On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret <
MDiCarlo <@t> kaleidahealth.org> wrote:

> Histonetters,
>
>
>
> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
> femur that they think is osteoporotic.  After decaling the cross section
> of the femur in 10% formic acid for 12 days, processing using xylene and
> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
> section.  The knife just skips over the bone.  I have soaked the bone
> with a 50/50 solution of fabric softener and still can't get a section.
> Does anyone have any suggestions?
>
>
>
> Thank you.
>
>
>
> Peggy DiCarlo HT (ASCP)
>
> Orthopedic Bone Lab
>
> Buffalo General Hospital
>
> Buffalo, NY  14203
>
> 716-859-1293
>
>
>
>
>
> The Keeping You Informed section of Kaleida Health’s website features a
> wealth of information, stories and pictures about our valued workforce and
> about the tremendous momentum our organization is experiencing. Check us
> out at: www.kaleidahealth.org/kyi
>
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
> or previous e-mail messages attached to it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, or a person responsible for
> delivering it to the intended recipient, you are hereby notified that any
> further review, disclosure, copying, dissemination, distribution, or use of
> any of the information contained in or attached to this e-mail transmission
> is strictly prohibited. If you have received this message in error, please
> notify the sender immediately by e-mail, discard any paper copies, and
> delete all electronic files of the message. If you are unable to contact
> the sender or you are not sure as to whether you are the intended
> recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
Question: Are rhinos  overweight unicorns?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet