[Histonet] difficult cross section to cut

[DiCarlo, Margaret]

Histonetters,

For everyone that responded to my email, I was finally able to cut a
couple of sections of the cross section of the femur.  I surface soaked
the block with 50/50 solution of fabric softener for TWO hours,
constantly soaking the gauze so it wouldn't dry out, covered it with a
plastic container inverted and then iced it for 5 minutes before taking
a section.  Also, I used a heavy duty long, 25 cm. steel c profile blade
to cut the bone.  I don't know if it made a difference but I had
previously used a shorter, 18.5 cm long steel c knife.  Now the trick
will be to see if the section stays on when I stain it.

Thanks for everyone's help.

Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY  14203
716-859-1293

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DiCarlo,
Margaret
Sent: Tuesday, January 10, 2012 12:35
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] difficult cross section to cut

Histonetters,

 

I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic.  After decaling the cross section
of the femur in 10% formic acid for 12 days, processing using xylene and
embedding in Tissue Prep 2, I have been unsuccessful in attaining a
section.  The knife just skips over the bone.  I have soaked the bone
with a 50/50 solution of fabric softener and still can't get a section.
Does anyone have any suggestions?  

 

Thank you.

 

Peggy DiCarlo HT (ASCP)

Orthopedic Bone Lab

Buffalo General Hospital

Buffalo, NY  14203

716-859-1293

 



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CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.