[Histonet] difficult cross section to cut

[Rene J Buesa]

Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10º, go to 30º angle.
René J.

--- On Tue, 1/10/12, DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org> wrote:


From: DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org>
Subject: [Histonet] difficult cross section to cut
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, January 10, 2012, 12:35 PM


Histonetters,



I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic.  After decaling the cross section
of the femur in 10% formic acid for 12 days, processing using xylene and
embedding in Tissue Prep 2, I have been unsuccessful in attaining a
section.  The knife just skips over the bone.  I have soaked the bone
with a 50/50 solution of fabric softener and still can't get a section.
Does anyone have any suggestions?  



Thank you.



Peggy DiCarlo HT (ASCP)

Orthopedic Bone Lab

Buffalo General Hospital

Buffalo, NY  14203

716-859-1293





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