[Histonet] difficult cross section to cut

[DiCarlo, Margaret]

Histonetters,

 

I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic.  After decaling the cross section
of the femur in 10% formic acid for 12 days, processing using xylene and
embedding in Tissue Prep 2, I have been unsuccessful in attaining a
section.  The knife just skips over the bone.  I have soaked the bone
with a 50/50 solution of fabric softener and still can't get a section.
Does anyone have any suggestions?  

 

Thank you.

 

Peggy DiCarlo HT (ASCP)

Orthopedic Bone Lab

Buffalo General Hospital

Buffalo, NY  14203

716-859-1293

 



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