[Histonet] Re: Histonet Digest, Vol 98, Issue 9

Sat Jan 7 22:04:29 CST 2012

Michele,
This is our procedure for toe & finger nails;
1) Pre-fix nails in 10% NFB as usual
2) Soak fixed nails in NAIR or any counter nail remover until soften
or bend easily (toe nail take longer; thickness dependent)
3) wash nail with water
4) Process in tissue processor
5) embed & cut
Note; we found soften with NAIR before processing work the best.

If NAIR is used after processing;
1) cut nail on charge slides (+)
2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly
3) bake jar in ~ 60c over for ~ 30 min
4) cool & open jar in fume hood ~ 5 min
5) stain as usual

Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org

On Sat, Jan 7, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
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>   1. finger nails (Michele Carr)
>   2. Temporary & Direct hire PA(ASCP) for February/March       (Refer a
>      Friend) (Cheryl)
>   3. Saffron (Gagnon, Eric)
>   4. Re: finger nails (Rene J Buesa)
>   5. Quality assurance program for pathologists (Diana McCaig)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
>        <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining.  The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?  Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
>
> ------------------------------
>
> Message: 2
> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
>        February/March  (Refer a Friend)
> To: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello 'Netters-
>
> Do you know any temp/travel PAs?  We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!!   If you refer someone we help, you get a referral bonus (keep it or share it with your friend!)
>
> 1. Temp traveler for at least 4 weeks in mid-February.
> 2. Direct Hire in 6 different institutions around the US.  Entry through Supervisory.
>
> As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide.
>
> Give me a call--make sure those you refer mention your name--I LIKE writing referral checks!
>
> Cheryl
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
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> ------------------------------
>
> Message: 3
> Date: Fri, 6 Jan 2012 15:19:18 -0500
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] Saffron
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Beth, as Bob Richmond has noted regarding saffron,
>
> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
> trichrome stain. It was in use as a general oversight stain in a few
> pathology services when I was a resident in the 1960's..."
>
> and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain.
>
> We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron.  Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store.  One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers.  Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change.
>
> I still think the wafting of the boiling saffron is quite a pleasant aroma.
>
> Eric Gagnon MLT
> Histology Laboratory
> Kingston General Hospital
> Kingston, Ontario, Canada
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
>        <Histonet <@t> lists.utsouthwestern.edu>,    Michele Carr
>        <michelecarr10 <@t> yahoo.com>
> Message-ID:
>        <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining.  The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?  Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 6 Jan 2012 17:06:19 -0500
> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] Quality assurance program for pathologists
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain;       charset="us-ascii"
>
> Is anyone willing to share with me their quality assurance/management
> program for pathologists.
>
>
>
> Sincere thanks
>
> Diana
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 98, Issue 9
> ***************************************