[Histonet] RE:decal solution for molecular studies

[Mayer,Toysha N]

Clare,

I just checked with our MB instructors and they said that the decal with EDTA should be ok, as long as you rinse it well after using, to remove the residual acid.
Hope that helps.



Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org
>
> ----------------------------------------------------------------------
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> Message: 1
> Date: Thu, 5 Jan 2012 10:16:06 -0500
> From: Clare Thornton <CThornton <@t> dahlchase.com>
> Subject: [Histonet] RE: decal solution for molecular studies
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> ? ? ? ?<histonet <@t> lists.utsouthwestern.edu>
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> To clarify, the Formical we use is a formic acid/EDTA solution.
>
>
> Clare J. Thornton, HTL(ASCP), QIHC
> Assistant Histology Supervisor
> Dahl-Chase Diagnostic Services
> 417 State Street, Suite 540
> Bangor, ME 04401
> cthornton <@t> dahlchase.com
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clare Thornton
> Sent: Thursday, January 05, 2012 9:12 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] decal solution for molecular studies
>
> Does anyone know of a decal solution that enables molecular studies (Her 2 FISH, EGFR by PCR) to be performed later? ?We use Formical for our decal solution. ?Her 2 FISH works about 50% of the time, EGFR almost never. ?We are looking at having to cut undecalcified bone today for both these studies, and we are not equipped to cut undecalcified bone. ?Any suggestions?
>
>
>
> Clare J. Thornton, HTL(ASCP), QIHC
> Assistant Histology Supervisor
> Dahl-Chase Diagnostic Services
> 417 State Street, Suite 540
> Bangor, ME 04401
> cthornton <@t> dahlchase.com
>
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