[Histonet] RE: Elastichrome paper, and troubleshooting

[Elizabeth Chlipala]

Tim

We do this stain all of the time, we never used the original reference we just made up one on our own.  I'll send our SOP in a different e-mail.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, January 05, 2012 10:45 AM
To: Histonet
Subject: [Histonet] Elastichrome paper, and troubleshooting

Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain:

Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975

We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past.

I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible.

Our problem is that the elastic stain washes out during or after the trichrome.

Procedure:
Bouins, 56C one hour
Wiegerts hematolylin, 3min
Verhoffs elastic stain, 15 min
2% ferric chloride differentiation (so far so-good)
Gomori's trichrome Blue, 15 min
0.2% glacial acetic acid, 1 min
Wash dH20, dehydrate, clear, mount

Results:  trichrome looks great, no elastin stain.

I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions.

Thanks for any help.


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>

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