[Histonet] RE: Elastichrome paper, and troubleshooting
liz <@t> premierlab.com
Thu Jan 5 11:50:05 CST 2012
We do this stain all of the time, we never used the original reference we just made up one on our own. I'll send our SOP in a different e-mail.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, January 05, 2012 10:45 AM
Subject: [Histonet] Elastichrome paper, and troubleshooting
Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain:
Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975
We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past.
I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible.
Our problem is that the elastic stain washes out during or after the trichrome.
Bouins, 56C one hour
Wiegerts hematolylin, 3min
Verhoffs elastic stain, 15 min
2% ferric chloride differentiation (so far so-good)
Gomori's trichrome Blue, 15 min
0.2% glacial acetic acid, 1 min
Wash dH20, dehydrate, clear, mount
Results: trichrome looks great, no elastin stain.
I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions.
Thanks for any help.
Supervisor, Histology, IPOX
UC San Francisco Medical Center
1600 Divisidero St, B217
San Francisco, CA 94115
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
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