[Histonet] Elastichrome paper, and troubleshooting

[Morken, Timothy]

Does anyone have a pdf (or could fax me) the original paper for "Elastichrome" stain:

Richardson, L. "Combination Elastic Trichrome Stain," Laboratory Medicine, 6.1, 1975

We have a procedure with this reference, but no original paper to look at. We do it very rarely and no one seems to know how well it worked in the past.

I do have another paper in the same vein, "A combination verhoeff's elastic and masson's trichrom stain for routine histology," O'Connor, S. Valle, Stain Technology V 57, no. 4, 1982, that is a modification of the Richardson paper, and gives us some ideas, but I would still like to see the original paper if possible.

Our problem is that the elastic stain washes out during or after the trichrome.

Procedure:
Bouins, 56C one hour
Wiegerts hematolylin, 3min
Verhoffs elastic stain, 15 min
2% ferric chloride differentiation (so far so-good)
Gomori's trichrome Blue, 15 min
0.2% glacial acetic acid, 1 min
Wash dH20, dehydrate, clear, mount

Results:  trichrome looks great, no elastin stain.

I'm thinking the acetic acid is too long so told them to shorten it to a few dips and see how it looks. But, maybe some has some experience with this and has other suggestions.

Thanks for any help.


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>