[Histonet] RE: Look at me...
liz <@t> premierlab.com
Thu Jan 5 11:36:16 CST 2012
If you are looking to stain cleaved caspase 3 there is a better antibody from cell signaling it works in multiple species, it's a bit pricey but I have found that it works the best out of the few that I have tried. We have used it mouse xenografts before without any issue.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, January 05, 2012 10:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Look at me...
I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts.
Thanks again =)
Sarah Goebel-Dysart, BA, HT(ASCP)
2150 Woodward Street
Austin, Texas 78744
(512)901-0900 ext. 6912
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