[Histonet] Using Nail Polish on coverslip?

[Kasai, Miki (NIH/NCI) [E]]

We are currently starting up some IHC on frozen tissue sections.  After staining with different fluorescent antibodies, we end with applying DAPI w/Prolong gold and then coverslipping.  We would like to seal the coverslip so that we can keep the slides longer.

Any suggestions on where and how best to apply the nail polish for a permanent fix on the coverslips?

Thanks,