[Histonet] RE: Paraffin blocks cracking

[Mayer,Toysha N]

Also, using 100% alcohol on the cold plate induces cracking. Freeze spray does too, although some people will never admit to using it. Both of these things induce the rapid cooling effect.

Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org
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Message: 8
Date: Wed, 4 Jan 2012 17:55:56 +0000 (UTC)
From: Pam  Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] Paraffin Blocks Cracking
To: Rene J Buesa <rjbuesa <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<1488794099.223852.1325699756078.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.net>
	
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I agree however; leaving blocks on the cold plate too long will also cause cracking of blocks even at -2C. 




----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com> 
To: histonet <@t> lists.utsouthwestern.edu, "Brian foster" <brian1975 <@t> email.com> 
Sent: Wednesday, January 4, 2012 11:50:13 AM 
Subject: Re: [Histonet] Paraffin Blocks Cracking 

Rapid cooling is the problem, but I do not think that -5??C can cause it. The effect is more noticeable in large but thin blocks. 
Ren?? J. 

--- On Wed, 1/4/12, Brian foster <brian1975 <@t> email.com> wrote: 


From: Brian foster <brian1975 <@t> email.com> 
Subject: [Histonet] Paraffin Blocks Cracking 
To: histonet <@t> lists.utsouthwestern.edu 
Date: Wednesday, January 4, 2012, 12:33 PM 


Hello all, 


I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? 
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End of Histonet Digest, Vol 98, Issue 4
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