[Histonet] Paraffin Blocks Cracking

[Pam Marcum]

I agree however; leaving blocks on the cold plate too long will also cause cracking of blocks even at -2C. 




----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com> 
To: histonet <@t> lists.utsouthwestern.edu, "Brian foster" <brian1975 <@t> email.com> 
Sent: Wednesday, January 4, 2012 11:50:13 AM 
Subject: Re: [Histonet] Paraffin Blocks Cracking 

Rapid cooling is the problem, but I do not think that -5ºC can cause it. The effect is more noticeable in large but thin blocks. 
René J. 

--- On Wed, 1/4/12, Brian foster <brian1975 <@t> email.com> wrote: 


From: Brian foster <brian1975 <@t> email.com> 
Subject: [Histonet] Paraffin Blocks Cracking 
To: histonet <@t> lists.utsouthwestern.edu 
Date: Wednesday, January 4, 2012, 12:33 PM 


Hello all, 


I am studying for my HTL and have a question that has been a problem for me and was wondering if anyione out there can help. "What cuases paraffin blocks to crack ?" So far i have come up with it has to do the crystal formation during cooling, and I have seen some Histonet articles that say the block cooling plate at the embedding may be too cold ( less than -5 C). Can anyone confirm that is the is the correct cause of the problem? 
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