[Histonet] Problem getting good sections from Biopsies and Liver
Rick and Sue Boen
rboen <@t> clearwire.net
Sat Feb 25 15:14:40 CST 2012
If you have access, read this article in the Journal of Histotechnology, Volume 34, Issue 2, pp 65-68, "Gastrointestinal Biopsies: More Action and Less "Chatter?". It's a good discussion about improving section quality of small biopsies.
Make sure your processing schedule is right.
Face the blocks slowly.
Avoid over aggressive facing.
Soaking the blocks on ice water after facing.
Use only sharp blades.
Section slowly at a constant speed.
Re-soak the block between levels.
Rick Boen, HTL (ASCP)
St. Luke's Hospital
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