[Histonet] Fire extinguishers for electronic equipment.
Stephen G. Ruby
Sruby <@t> 4path.com
Sat Feb 25 16:43:44 CST 2012
Halon extinguishers have been phased out.
There is now a newer version: Halotron
I really don't know the difference chemically.
Google : personal fire extinguisher computer equipment it came up with about 3 million hits.
With that you should have a huge number of companies which will have them for order.
Stephen G. Ruby, MD, MBA, FCAP
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Saturday, February 25, 2012 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 99, Issue 33
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Today's Topics:
1. staining of nerve tissue for confocal microscopy (Salomao Segal)
2. Removing tape (M.O.)
3. RE: Removing tape (Carol Bryant)
4. fire extinguishers (Blazek, Linda)
5. RE: fire extinguishers (Elizabeth Chlipala)
6. RE: fire extinguishers (Blazek, Linda)
7. RE: fire extinguishers (O'Donnell, Bill)
8. Fw: [Histonet] RE: fire extinguishers (Lee & Peggy Wenk)
9. Grossing techs (Bruce Gapinski)
10. RE: Grossing techs (Davide Costanzo)
11. PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY
(Wilson A)
12. Re: PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER
BIOPSY (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Fri, 24 Feb 2012 12:23:42 -0600
From: Salomao Segal <ssegal2 <@t> slu.edu>
Subject: [Histonet] staining of nerve tissue for confocal microscopy
To: histonet <@t> lists.utsouthwestern.edu
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Greetings!
I am looking for staining methods applicable to thick sections of nervous
tissue for confocal microscopy.
We would like to stain cell processes and use specialized software for
reconstruction of dendrites.
We do not have the capability to impale cells and impregnate them with for
example lucifer yellow.
Golgi?
Other methods?
Any information would be greatly appreciated.
Best wishes and thanks
--
Solomon Segal, M.D.
Assistant Professor of Anatomy in Surgery
Center for Anatomical Science and Education (CASE)
Department of Surgery
School of Medicine
Saint Louis University
1402 South Grand Blvd.
Schwitalla Hall - 3rd Floor - M310
Saint Louis, MO, 63104
office: 314 977 8023
laboratory: 314 977 8080
CASE: 314 977 8027
FAX: 314 977 5127
e-mail: ssegal2 <@t> slu.edu
http://medschool.slu.edu/anatomy/
http://slu.academia.edu/SolomonSegal
https://sites.google.com/a/slu.edu/segal-laboratory/
https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/
------------------------------
Message: 2
Date: Fri, 24 Feb 2012 10:29:49 -0800
From: "M.O." <modz9636 <@t> gmail.com>
Subject: [Histonet] Removing tape
To: histonet <@t> lists.utsouthwestern.edu
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Dear All,
I am using clear packing tape on mma embedded rabbit femurs with implants.
The reasoning for the use of tape is that the implant crumbles when being
cut without the tape. I have switched to a new tape that partially comes
off with xylene, but does not release from the section on the slide (held
with haupts). Does anyone have suggestions for removing the tape - xylene
vs toluene vs acetone? At this point, the slides have been in xylene
overnight and have not been released from the slides and the other tape
took 45mins to lift off.
Thank you so much,
Merissa
------------------------------
Message: 3
Date: Fri, 24 Feb 2012 13:52:51 -0500
From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: RE: [Histonet] Removing tape
To: 'M.O.' <modz9636 <@t> gmail.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF41207CD485 <@t> EXCHANGESB>
Content-Type: text/plain; charset="us-ascii"
Please reply to all. I am interested in this info. also.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of M.O.
Sent: Friday, February 24, 2012 1:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Removing tape
Dear All,
I am using clear packing tape on mma embedded rabbit femurs with implants.
The reasoning for the use of tape is that the implant crumbles when being
cut without the tape. I have switched to a new tape that partially comes
off with xylene, but does not release from the section on the slide (held
with haupts). Does anyone have suggestions for removing the tape - xylene
vs toluene vs acetone? At this point, the slides have been in xylene
overnight and have not been released from the slides and the other tape
took 45mins to lift off.
Thank you so much,
Merissa
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Message: 4
Date: Fri, 24 Feb 2012 14:49:22 -0500
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] fire extinguishers
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com<mailto:lblazek <@t> digestivespecialists.com>
------------------------------
Message: 5
Date: Fri, 24 Feb 2012 12:54:40 -0700
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: fire extinguishers
To: "'Blazek, Linda'" <lblazek <@t> digestivespecialists.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<14E2C6176416974295479C64A11CB9AE011390CC583A <@t> SBS2K8.premierlab.local>
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Linda
We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, February 24, 2012 12:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fire extinguishers
Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com<mailto:lblazek <@t> digestivespecialists.com>
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------------------------------
Message: 6
Date: Fri, 24 Feb 2012 16:01:58 -0500
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] RE: fire extinguishers
To: 'Elizabeth Chlipala' <liz <@t> premierlab.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E39137DA0215D <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
Thanks Liz,
The guy that was in here today said we should use the Halon or Ansul Cleanguard extinguishers on any piece of equipment to reduce the damage from the ABC extinguishers. I know we have Halon in the server room but I've never heard of having one in the lab itself. He was also talking about a price of $350 - $400.
-----Original Message-----
From: Elizabeth Chlipala [mailto:liz <@t> premierlab.com]
Sent: Friday, February 24, 2012 2:55 PM
To: Blazek, Linda; histonet <@t> lists.utsouthwestern.edu
Subject: RE: fire extinguishers
Linda
We do, we have it located where our server is, its only supposed to be used on our server, we have regular A B C extinguishers in the lab. The Halon ones are for computers and stuff like that. They are not inexpensive, they cost about $185.00 each. They are liquid that does not damage the computer equipment.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, February 24, 2012 12:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fire extinguishers
Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab? The company that does our sprinkler system has suggested that we have that kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com<mailto:lblazek <@t> digestivespecialists.com>
_______________________________________________
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------------------------------
Message: 7
Date: Fri, 24 Feb 2012 14:27:25 -0700
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: RE: [Histonet] fire extinguishers
To: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4940DF6D1C5FDF48931B6966AAEF9395479063 <@t> chimsx08.CHI.catholichealth.net>
Content-Type: text/plain; charset="ISO-8859-1"
Use of halon extinguishers.... Do not used in a small or closed space.
The way it works is by rapidly depleting oxygen available for the fire.
May well do the same for you.
Just sayin...... Machinery is replacable.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, February 24, 2012 1:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fire extinguishers
Does anyone have a Halon or Ansul Clean guard fire extinguisher in the
lab? The company that does our sprinkler system has suggested that we
have that kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton
Phone: (937) 396-2623
Email:
lblazek <@t> digestivespecialists.com<mailto:lblazek <@t> digestivespecialists.com
>
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Message: 8
Date: Fri, 24 Feb 2012 16:30:34 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Fw: [Histonet] RE: fire extinguishers
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E5C136A4B98A45EFADF91112964346BD <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Be careful with Halon. We at Ford used in in our server rooms, but we had
many hundreds of thousands of dollars of servers and a fairly large room.
If it is set off, EVERYBODY MUST leave the room. Halon will NOT support
life
(this is why it works as a fire suppressor). So, along with the Halon, you
should have a good way to flush the Halon from the room before returning to
it.
Lee & Peggy Wenk
Actually I'm Lee, not Peggy.
-----Original Message-----
From: Blazek, Linda
Sent: Friday, February 24, 2012 4:01 PM
To: 'Elizabeth Chlipala' ; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: fire extinguishers
Thanks Liz,
The guy that was in here today said we should use the Halon or Ansul
Cleanguard extinguishers on any piece of equipment to reduce the damage from
the ABC extinguishers. I know we have Halon in the server room but I've
never heard of having one in the lab itself. He was also talking about a
price of $350 - $400.
-----Original Message-----
From: Elizabeth Chlipala [mailto:liz <@t> premierlab.com]
Sent: Friday, February 24, 2012 2:55 PM
To: Blazek, Linda; histonet <@t> lists.utsouthwestern.edu
Subject: RE: fire extinguishers
Linda
We do, we have it located where our server is, its only supposed to be used
on our server, we have regular A B C extinguishers in the lab. The Halon
ones are for computers and stuff like that. They are not inexpensive, they
cost about $185.00 each. They are liquid that does not damage the computer
equipment.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, February 24, 2012 12:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fire extinguishers
Does anyone have a Halon or Ansul Clean guard fire extinguisher in the lab?
The company that does our sprinkler system has suggested that we have that
kind.
Thanks,
Linda
Our Vision: To be the #1 choice for all your GI services Linda Blazek HT
(ASCP) Manager/Supervisor GI Pathology of Dayton
Phone: (937) 396-2623
Email:
lblazek <@t> digestivespecialists.com<mailto:lblazek <@t> digestivespecialists.com>
_______________________________________________
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------------------------------
Message: 9
Date: Fri, 24 Feb 2012 15:52:34 -0600
From: "Bruce Gapinski" <BGapinski <@t> pathgroup.com>
Subject: [Histonet] Grossing techs
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<197CD0B02A81F94994A285C59C8AE05C083E8B6568 <@t> pgnexchange.pathgroup.com>
Content-Type: text/plain; charset=us-ascii
We are looking for help with our grossing. Can you help? You must be CLIA qualified to do the high complexity testing we know as grossing. Times have changed as we used to do this work ourselves, but no more. Two of my staff qualify but I need another.
We are a small laboratory in Marin County, just north of SF.
Respectfully,
Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF
________________________________
Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you
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Message: 10
Date: Fri, 24 Feb 2012 14:01:09 -0800
From: Davide Costanzo <pathlocums <@t> gmail.com>
Subject: RE: [Histonet] Grossing techs
To: Bruce Gapinski <BGapinski <@t> pathgroup.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2522340837944687498 <@t> unknownmsgid>
Content-Type: text/plain; charset=ISO-8859-1
Preserve the profession - hire an ASCP Certified PA.
Sent from my Windows Phone
From: Bruce Gapinski
Sent: 2/24/2012 1:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Grossing techs
We are looking for help with our grossing. Can you help? You must be
CLIA qualified to do the high complexity testing we know as grossing.
Times have changed as we used to do this work ourselves, but no more.
Two of my staff qualify but I need another.
We are a small laboratory in Marin County, just north of SF.
Respectfully,
Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF
________________________________
Important Notice: This e-mail is intended for the use of the person to
whom it is addressed and may contain information that is privileged
and confidential. If you are not the intended recipient, any
disclosure, copying, distribution, or use of the contents of this
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error, please destroy this message and contact the Security Officer at
PathGroup, Inc immediately at 615-562-9255. Thank you
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------------------------------
Message: 11
Date: Fri, 24 Feb 2012 19:28:55 -0800 (PST)
From: Wilson A <wilson6848 <@t> yahoo.com>
Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND
LIVER BIOPSY
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1330140535.4261.YahooMailNeo <@t> web120903.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
?
??? Hi,
?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation.
?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem.
?? Hoping to read from you guys asap. You guys are the best.
?
Thanks,
?
Wilson
------------------------------
Message: 12
Date: Sat, 25 Feb 2012 08:34:35 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES
AND LIVER BIOPSY
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Wilson A <wilson6848 <@t> yahoo.com>
Message-ID:
<1330187675.4050.YahooMailClassic <@t> web162104.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration".
"Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF.
If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration".
After that many "good" conditions I am sure you will end with a "good section".
Check all the steps in your work flow and check if all steps are as "good" as they should.
Ren? J.
--- On Fri, 2/24/12, Wilson A <wilson6848 <@t> yahoo.com> wrote:
From: Wilson A <wilson6848 <@t> yahoo.com>
Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, February 24, 2012, 10:28 PM
?
??? Hi,
?????? Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation.
?? I will really, really appreciate if? you guys in histoland could suggest some solutions that could stop the problem.
?? Hoping to read from you guys asap. You guys are the best.
?
Thanks,
?
Wilson
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