[Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Feb 25 10:34:35 CST 2012
To guarantee a "good section" two main issues have to be resolved: "good fixation" leading to "good infiltration".
"Good fixation" depends on the slices of tissue being as thin as possible and fixed during the adequate time to assure a complete fixation, especially if you use NBF.
If the "good fixation" is achieved, then your processing protocol has to assure a "good infiltration".
After that many "good" conditions I am sure you will end with a "good section".
Check all the steps in your work flow and check if all steps are as "good" as they should.
--- On Fri, 2/24/12, Wilson A <wilson6848 <@t> yahoo.com> wrote:
From: Wilson A <wilson6848 <@t> yahoo.com>
Subject: [Histonet] PROBLEM GETTING GOOD SECTIONS FROM BIOPSIES AND LIVER BIOPSY
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, February 24, 2012, 10:28 PM
Please we are having problems getting great slides from tissue/biopsy specimens like gastric, esophageal, needdle biopsy and Liver Biopsy. Our pathologists are not happy at all because of this situation.
I will really, really appreciate if you guys in histoland could suggest some solutions that could stop the problem.
Hoping to read from you guys asap. You guys are the best.
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