[Histonet] questions

Kim Donadio one_angel_secret <@t> yahoo.com
Wed Feb 22 17:51:17 CST 2012

Dorothy, saving cut slides forever is not a good idea in my opinion. They attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen specificity.  Cut slides is not the answer to me. I had to deal with this a couple if times hopefully your pathologist will understand it's best to leave the cells in the block till need. Now I will say on certain protocols we would take  sections and stain them but really need to get out of habit of cutting a bunch of extras that even if you did need would be sub optimal material because of what we just spoke of. Keep it in the block as much as you can. Just my two cents. 
Kim D

Sent from my iPhonem 

On Feb 22, 2012, at 1:18 PM, "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:

> For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc??
> How long are unstained slides usable?  Do any of you pick up extra sections from a ribbon if the tissue is minimal ?  We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block.
> Appreciate ahead of time your responses!
> Dorothy Webb, HT (ASCP)
> Regions Hospital
>  ________________________________
> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
> If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list