one_angel_secret <@t> yahoo.com
Wed Feb 22 17:51:17 CST 2012
Dorothy, saving cut slides forever is not a good idea in my opinion. They attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen specificity. Cut slides is not the answer to me. I had to deal with this a couple if times hopefully your pathologist will understand it's best to leave the cells in the block till need. Now I will say on certain protocols we would take sections and stain them but really need to get out of habit of cutting a bunch of extras that even if you did need would be sub optimal material because of what we just spoke of. Keep it in the block as much as you can. Just my two cents.
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On Feb 22, 2012, at 1:18 PM, "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
> For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc??
> How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block.
> Appreciate ahead of time your responses!
> Dorothy Webb, HT (ASCP)
> Regions Hospital
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