[Histonet] RE: questions

Debra Siena DSiena <@t> statlab.com
Wed Feb 22 17:35:54 CST 2012

Hi Dorothy,

I have used reagent alcohol for years both with staining and tissue processing.  I have never noticed any artifacts or crystals due to use of reagent alcohol.  One thing that you should know is that all denatured alcohols are not the same so make sure that you buy a denatured (reagent) alcohol of good quality.

As far as keeping slides unstained forever, the one thing that you need to take into consideration is that if the slide is charged or silane coated that the positive charges (adhesiveness) of the slide will change over time and the sections may fall off during staining.  Also the antigenicity of some antibodies decreases over time so if you were to do IHC staining, your results may not be as strong as they would be on a more recently cut slide.

Best wishes,

Debbie Siena HT (ASCP) QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010  x229 | Fax: 972-436-1369
dsiena <@t> statlab.com | www.statlab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Wednesday, February 22, 2012 12:18 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions

For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc??

How long are unstained slides usable?  Do any of you pick up extra sections from a ribbon if the tissue is minimal ?  We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block.

Appreciate ahead of time your responses!

Dorothy Webb, HT (ASCP)
Regions Hospital

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