[Histonet] questions

Mark Bromley Mark.Bromley <@t> mps.com.au
Wed Feb 22 18:06:30 CST 2012

Hi Dorothy

If you want to prolong the viability of pre-cut unstained slides,
firstly dry them, then dunk the slide into a bath of molten paraffin wax
deep enough to submerge all of the tissue section, then remove it and
allow the wax coating to solidify. This will keep the environment out of
your cut section. When you need to use the slides, simply heat to melt
the excess wax and then dewax and rehydrate in the usual manner.


Mark Bromley. Manager, Histopathology.
Melbourne Pathology. 103 Victoria Pde, Melbourne. | (03) 92877806 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Thursday, 23 February 2012 5:18 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions

For those of you who use reagent alcohol, have you ever experienced any
problems in processing or staining, such as artifacts, crystals forming,

How long are unstained slides usable?  Do any of you pick up extra
sections from a ribbon if the tissue is minimal ?  We do and have used
them at times, but a pathologist would like them saved "forever" in case
tissue is needed for a molecular test and there is not enough left in
the block.

Appreciate ahead of time your responses!

Dorothy Webb, HT (ASCP)
Regions Hospital

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