AW: [Histonet] C4d IF on FFPE kidney

Tony Reilly Tony_Reilly <@t>
Thu Feb 2 17:14:24 CST 2012

Hi Gudren
We have been doing both IF and IHC for C4d for quite a while.  While we
have found that the IF staining is superior to the IHC we are not
unhappy with the IHC results.  Our IHC results have improved since
getting a Ventana Ultra which has allowed better control of the

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Clinical and Statewide Services Division| QueenslandHealth
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t>

>>> "Gudrun Lang" <gu.lang <@t>> 2/3/2012 12:31 am >>>
We have been performing C4d IHC on FFPE kidney for a couple of years.
receive only fixed samples.
Yesterday my doctors came with this idea of IF - I still have to ask
for the
special reasons. But I have the suspicion, that they are not aware of
fact, that IF on frozen unfixed tissue is the usual way found in

There are some articles that deal with comparison of IF(frozen) and
IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows
additional staining in glomeruli.

Perhaps someone told them, that IF is the golden standard and


-----Ursprüngliche Nachricht-----
Von: Richard Cartun [mailto:Rcartun <@t>] 
Gesendet: Mittwoch, 01. Februar 2012 21:21
An: gu.lang <@t> 
Betreff: Re: [Histonet] C4d IF on FFPE kidney

Hi Gudrun:

Is there reason why you want to use IF and not immunoperoxidase?


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> "Gudrun Lang" <gu.lang <@t>> 2/1/2012 3:17 PM >>>

Can someone provide me a immunofluorescence protocol for C4d on
fixed human paraffin sections?

thanks in advance

Gudrun Lang

Histonet mailing list
Histonet <@t> 

Histonet mailing list
Histonet <@t>

This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error.
Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.  The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters.
If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.  You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced.
If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited.
Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email.
Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government.

More information about the Histonet mailing list