[Histonet] Melanin Bleaching

[Janci Wellborn]

I am looking for alternatives to the potassium permanganate melanin bleaching procedure when doing IHC. I have seen literature references to doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa counterstain in the past. Does any one have either procedure that they would be willing to share? 
 
We are a small volume lab and the costs associated with bringing in a different color IHC kit is not justified. 
 
Any help would be greatly appreciated.
 
Janci
 
 
 
Janci R Wellborn, HTL (ASCP)
Laboratory Technical Coordinator
Histology
Alton Memorial Hospital
1 Memorial Drive
Alton, Ill 62002
618-463-7082