[Histonet] MDM2 Probe/Ventana

[Mark Tarango]

Hi Vicki,

I don't have a protocol but it should be very similar to the HER2 dual ISH.
 I'm assuming that you'll be using a probe targeted to chromosome 12 too
and not just doing the MDM2 (but maybe I'm wrong).  I would start there.

I'm working on validating MDM2 FISH and it's nearly identical to our HER2
FISH protocol, right down to denaturation and hybridization times.

Mark

On Wed, Dec 26, 2012 at 6:05 AM, Gauch, Vicki <GauchV <@t> mail.amc.edu> wrote:

> Hi,
> Does anyone have a protocol for the MDM2 Probe from Ventana that they
> could share ?  We have been unable to locate one thus far and our IHC
> Technical Specialist needs one.
> Thank you in advance for your assistance,
>
> Vicki Gauch
> AMCH
> Albany, NY
>
>
>
>
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