[Histonet] Trichrome

[Markus F. Meyenhofer]

Right, I bet these biopsies were in fine mesh/holes tissue capsules and air 
has little or no escape, so tissues or parts of tissues were not in all 
solutions during fixing/ processing!
----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Cheryl" <tkngflght <@t> yahoo.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, December 04, 2012 1:26 PM
Subject: Re: [Histonet] Trichrome


IF you are not successful when the slides are stained on the Nexus OR 
manually, while the controls stain well, you can eliminate the instrument as 
the cause.
It is most likely a processing artifact: either incomplete fixation, or 
overdehydration, or incomplete dewaxing.
Look to your protocol instead to your instrument.
René J.

From: Cheryl <tkngflght <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 4, 2012 8:18 AM
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers 
is inconsistent. Taking it off the stainer and doing it by hand with a kit 
has similar results.

The tissue is NBF fixed needle biopsies on kidney. There is a little 
mechanical damage on the edges of the tissue but the collagens are staining 
too light and the red overstains. The normal tissue as control is 
beautiful -- I know I know--don't force the stain--but it doesn't make sense 
to have this much variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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