[Histonet] Trichrome

[Tom McNemar]

Same problem here.  Using the Nexus stainer and have also experienced this with the Reticulin as well.  Called service today and they elected to send an application specialist instead of a service tech.  Will see what happens.  Would not have expected to see this when doing the stain by hand....

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is inconsistent.  Taking it off the stainer and doing it by hand with a kit has similar results.

The tissue is NBF fixed needle biopsies on kidney.  There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.  The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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