[Histonet] Trichrome

Tom McNemar TMcNemar <@t> lmhealth.org
Tue Dec 4 11:53:12 CST 2012


Same problem here.  Using the Nexus stainer and have also experienced this with the Reticulin as well.  Called service today and they elected to send an application specialist instead of a service tech.  Will see what happens.  Would not have expected to see this when doing the stain by hand....

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is inconsistent.  Taking it off the stainer and doing it by hand with a kit has similar results.

The tissue is NBF fixed needle biopsies on kidney.  There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.  The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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