[Histonet] Trichrome

[Walter Benton]

Sounds like you may have a cracked vortex mixer and/or silver in your reagent dispensing line. If the later is the case new tubing will fix this issue and new vortex mixer(s) will fix the issue with mixing. Do you know how to perform a vortex mix test using the service menu functions? If so, place a drop of hematoxylin on a slide and with the lid open you will be able to see if the vortex mixers are blowing the solution counter-clockwise and clockwise. Each mixer does it in a different manner to make sure the reagents are mixed well over the entire slide.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wbenton <@t> cua.md
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar [TMcNemar <@t> lmhealth.org]
Sent: Tuesday, December 04, 2012 12:53 PM
To: 'Cheryl'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Trichrome

Same problem here.  Using the Nexus stainer and have also experienced this with the Reticulin as well.  Called service today and they elected to send an application specialist instead of a service tech.  Will see what happens.  Would not have expected to see this when doing the stain by hand....

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is inconsistent.  Taking it off the stainer and doing it by hand with a kit has similar results.

The tissue is NBF fixed needle biopsies on kidney.  There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.  The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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