[Histonet] Trichrome

[Cheryl]

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is inconsistent.  Taking it off the stainer and doing it by hand with a kit has similar results.
 
The tissue is NBF fixed needle biopsies on kidney.  There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.  The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer...
 
Help?


Cheryl Kerry, HT(ASCP) 
Path Group of Louisiana