[Histonet] DAPI staining

[Barone, Carol]

Someone sent me a question regarding DAPI staining ( I generally  use Hoechst )...but anyway, they are having smearing of nuclear contents. I would guess that is from rupturing of the nuclear membrane....over fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 mins to air dry. What do the experts out there say? This could be a learning moment for me , as well.